Fig 2: Screening and enrichment analysis of SPINT1/2 co-expressed genes by Linkedomics. Volcano plot of SPINT1 co-expressed genes (A). Top 50 positively (B) and top 50 negatively (C) correlated genes of SPINT1. Volcano plot of SPINT2 co-expressed genes (D). Top 50 positively (E) and top 50 negatively (F) correlated genes of SPINT2. Biological process (G) and KEGG pathway (H) enrichment of the SPINT1 co-expressed genes. Biological process (I) and KEGG pathway (J) enrichment of the SPINT2 co-expressed genes.

Fig 3: SPINT1/2 were correlated with patient prognosis. Correlation between SPINT1 expression and OS (A), RFS (B) and DMFS (C) from Kaplan–Meier plotter. Association between SPINT2 expression and OS (D), RFS (E) and DMFS (F) Kaplan–Meier plotter. The correlation between SPINT1 expression and the OS from GSE9893 (G), RFS from GSE1378 (H), DFS from GSE4922 (I) and DSS from GSE3494 (J). The correlation between SPINT2 expression and the OS from GSE9893 (K) and the RFS from GSE1379 (L) on PrognoScan server.

Fig 4: Methylation of SPINT1/2 was correlated with prognosis in breast cancer from MethSurv. Heatmaps of the association between methylation level of SPINT1 (A) and SPINT2 (B) and patient characteristics and genomic subregions. Association of methylation at TSS200; 5’UTR-cg11701759 (C), 3’UTR-Open_Sea-cg04519327 (D) and TSS200; 5’UTR-Island-cg27510007 (E) in SPINT1 with patient OS. Correlation between methylation at TSS1500-Island-cg10154122 (F) and TSS1500-N_Shore-cg22522066 (G) in SPINT2 and OS.

Fig 5: SPINT1/2 were overexpressed in breast cancer. (A) datasets reporting the upregulated expression of SPINT1/2 in Oncomine. The differential expression of SPINT1 mRNA in non- (B) and paired (C) breast cancer tissues. The mRNA expression of SPINT2 in non- (D) and paired (E) breast cancer samples. The protein expression of SPINT1 (F) and SPINT2 (G, H) in normal and cancer tissues from HPA database. *P <.05; **P <.01; ***P <.001.